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Characterization of Blood Mononuclear Phagocytes in Phrynops hilarii (Chelonia Chelidae) International Journal of Morphology
Pitol,Dimitrius Leonardo; Issa,Joáo Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The localization of peroxidase activity in different cell regions is used as a criterion for the classification of the stage of maturation of mammalian mononuclear phagocytes with a positive peroxidase reaction indicating the presence of monoblasts, promonocytes, monocytes and macrophages. In this study it was evaluated the peroxidase activity of blood mononuclear phagocytes of this turtle detected at different stages of differentiation. The present observations suggest that, in turtles, the differentiation of mononuclear phagocytes occur in the blood circulation, in contrast to animals, where only are monocytes in circulating blood and macrophage differentiation occurs in other body compartments
Tipo: Journal article Palavras-chave: Mononuclear phagocytes; Blood; Turtle.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200021
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Decalcification Dynamic of Dog Mineralized Tissue by Microwaves International Journal of Morphology
Pitol,Dimitrius Leonardo; Issa,Joáo Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The aim of this article is to present the decalcification process dynamic of mineralized tissue in dogs, teeth and jaw, comparing the traditional decalcification method, immersion, and microwave, immersion followed by irradiation using a domestic microwave oven, accompanying the liberation of calcium through spectrophotometer of atomic absorption. It was used as decalcified agent, EDTA solution or nitric acid. The results showed that with the use of nitric acid (5%), after 15 days, the irradiated fragments could be processed for histological analysis, otherwise the tooth not irradiated need to be submerged for 65 days. The EDTA decalcified action was slower than the nitric acid. The histological observations of the irradiated samples showed an excellent...
Tipo: Journal article Palavras-chave: Dog; Mineralized tissue; Decalcification; Microwaves; Solution.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200011
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Microwave Fixation in Rat Fetuses Tissues: Histological and Immunohistochemical Analysis International Journal of Morphology
Pitol,Dimitrius Leonardo; Mardegan Issa,João Paulo Mardegan; Camacho,Eduardo; Wolga,Nilce de Oliveira; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The aim of this study was to show the microwaves action in fixation of rat fetuses, dermal and cartilaginous tissues, using histological and immunohistochemistry methods for analysis. It was possible to conclude in this study using the rat as experimental model that the two methods for antibody retrieval, presented an excellent ways for the use of Ki67 antibody in the immunohistochemical analysis
Tipo: Journal article Palavras-chave: Microwave; Rat; Histological and immunohistochemical analysis; Fixation.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400005
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Morphological Characterization of the Leukocytes in Circulating Blood of the Turtle (Phrynops hilarii) International Journal of Morphology
Pitol,Dimitrius Leonardo; Issa,Joao Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The Phrynops hilarii specie of turtle has its characterization not well defined in the literature, it was proposed in this study the leukocyte characterization of the blood, stained by Leishman and analyzed under light and transmission electron microscope. It was not observe any cellular type with similar characteristics to neutrophils in mammalian group. We believed, based on the data obtained in this study that the heterophils have a morphofuncional analogy with another neutrophils belonged to mammalian group. This conclusion is being supported in many recent studies found in the literature
Tipo: Journal article Palavras-chave: Turtle; Morphology; Leukocytes; Blood.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400002
Registros recuperados: 4
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